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应用文章
Introduction
This application note presents a brief study of the mechanical properties of red blood cells (RBC) using the Keysight Technologies, Inc. NanoVision nanomechanical microscopy option. NanoVision delivers quantitative imaging by coupling a linear electromagnetic actuation-based indentation head with a closed-loop nanopositioning stage on the Keysight G200 Nano Indenter.
Sample Preparation and Imaging
A blood smear was prepared on a microscope slide and was dried for one hour before testing. The microscope slide was then mounted as a regular sample in a Keysight Nano Indenter G200.
Next, the sample is translated in X and Y under a diamond tip. A small load is applied to the tip in order to keep it in contact with the surface. The height variations recorded by the tip create the topographic image of the sample’s surface. The piezo-actuated sample stage used in the NanoVision option provides high accuracy in lateral positioning (2 nm) and imaging of the samples. A cubecorner diamond tip is utilized to image the surface profile of the sample.
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